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KMID : 0363219940320020234
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Korean Journal of Dermatology
1994 Volume.32 No. 2 p.234 ~ p.244
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Effects of Mycobacterium leprae, M. Lepra Phenolic Glycolipid-1 and Cytokines on the Nitric Oxide Generation of RAW 264.7 Macrophages
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박석돈
이재승/이복수/전창덕/정헌택/김종구
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Abstract
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Background:
@EN Nitric oxide(NO·)has been reproted to play an important role in macrophage-mediated microbicidal capacity for a variety of intracellular pathogens. NO·generation is used as an indicator of microbicidal function of macrophages.
@ES Objective:
@EN Our purpose is to investigate the production of NO ·from macrophages phagocytized tiwh Mycobacterium leprae or M. leprae phenoic glycolipid-1(PGL-1) for the purpose of elucidating the pathogenesis of leprosy.
@ES Methods:
@EN We used a murine macrophage cell line, RAW 264.7. Macrophages were incubated with dead M. leprae or PGL-1, respectively and then treated with interferon-gamma(IFN-γ) and /or tumor necrosis factor-alpha(TNF-α). The release of NO·was
determined
spectrophotometrically by measuring nitrite.
@ES Results:
@EM M. leprae and PGL-1 failed to stimulate NO·secretion except at high bacteria-to-cell rations(50:1)and at the highest concentration(100㎍/ml) of PGL-1. IFN-γor IFN-γplus TNF-αmarkedly stimulated macrophages phagocytized with M. leprae or
PGL-1 to
release NO·.
@ES Conclusion:
@EN Defective IFV-γ-dependent NO·production of macrophages may be an important factor in the pathogenesis of leprosy. (Kor J Dermatol 1994 ; 32(1) : 234~244)
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KEYWORD
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